Journal:

Article Title: Cardiac-type EC-Coupling in Dysgenic Myotubes Restored with Ca 2+ Channel Subunit Isoforms α 1C and α 1D Does not Correlate with Current Density

doi:

Figure Lengend Snippet: Properties of Ca 2+ currents and Ca 2+ transients recorded in reconstituted dysgenic myotubes with combined patch-clamp and fluo-4 Ca 2+ measurements

Article Snippet: When dysgenic myotubes are reconstituted with the cardiac Ca 2+ channel α 1C subunit, “cardiac-type” EC-coupling is restored, which is characterized by its dependence on Ca 2+ influx ( Tanabe et al., 1990 ).

Techniques: Patch Clamp

Journal:

Article Title: Cardiac-type EC-Coupling in Dysgenic Myotubes Restored with Ca 2+ Channel Subunit Isoforms α 1C and α 1D Does not Correlate with Current Density

doi:

Figure Lengend Snippet: Properties of action potential-induced Ca 2+ transients recorded in reconstituted dysgenic myotubes with fluo-4 Ca 2+ measurements

Article Snippet: When dysgenic myotubes are reconstituted with the cardiac Ca 2+ channel α 1C subunit, “cardiac-type” EC-coupling is restored, which is characterized by its dependence on Ca 2+ influx ( Tanabe et al., 1990 ).

Techniques:

Journal: Biophysical Journal

Article Title: A Role of BK Channel in Regulation of Ca 2+ Channel in Ventricular Myocytes by Substrate Stiffness

doi: 10.1016/j.bpj.2017.01.036

Figure Lengend Snippet: Substrate stiffness regulates protein and mRNA expression of the BK channel α- and β1-subunits. (A) Representative western blots showing BK channel α- and β1-subunit proteins in ventricular myocytes cultured on the soft and stiff substrates. (B) Summary of the mean protein expression from four independent experiments, normalized to that in cells on the soft and stiff substrate. (C) RT-PCR analysis of the mRNA expression of α- and β1-subunits in cells on the soft and stiff substrates. (D) Summary of the mean mRNA expression from four independent experiments, normalized to that in cells on the soft and stiff substrates. (E) RT-PCR analysis of the BK STREX-containing α-subunit in cells on the soft and stiff substrates. (F) Summary of the mRNA expression for the BK STREX-containing α-subunit from four independent experiments, normalized to that in cells on the soft and stiff substrates. ∗p < 0.05.

Article Snippet: The forward and reverse primer sequences used are: 5′-CCTGAGAAGGGAGTGGGAGACC-3′ and 5′-ATGTTGAGTGACGCCAAGATGC-3′ for the BK channel α -subunit with or without STREX; 5′-AGCCGAGCATGTTGTTTTGAT-3′ and 5′-ACGCACACGGCCTGACA-3′ for the STREX-containing α -subunit; 5′-TGTGCTGTCATCACCTACT-3′ and 5′-CATGGCAATAATGAGGAG-3′ for the BK channel β 1 -subunit.

Techniques: Expressing, Western Blot, Cell Culture, Reverse Transcription Polymerase Chain Reaction